Sphingomyelin turnover induced by vitamin D3 in HL-60 cells
作者： T Okazaki , R M Bell , Y A Hannun
DOI: 10.1016/S0021-9258(19)47268-2
关键词: Endocrinology 、 Cellular differentiation 、 Vitamin 、 Internal medicine 、 Ceramide 、 ENPP7 、 Sphingomyelin 、 Sphingolipid 、 Signal transduction 、 Phosphorylcholine 、 Biology 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Abstract Sphingolipid metabolism was examined in human promyelocytic leukemia HL-60 cells. Differentiation of cells with 1 alpha, 25-dihydroxyvitamin D3 (vitamin D3; 100 nM) accompanied by sphingomyelin turnover. Maximum turnover [3H]choline-labeled occurred 2 h following vitamin treatment, levels decreasing to 77 +/- 6% control and returning base-line 4 h. Ceramide phosphorylcholine were concomitantly generated. mass increased 55% at treatment returned The amount produced equaled the hydrolyzed, suggesting involvement a sphingomyelinase. Vitamin resulted 90% increase activity neutral sphingomyelinase from inferred role hydrolysis induction cell differentiation investigated using an exogenous When bacterial added concentrations that caused similar degree as nM D3, it enhanced ability subthreshold induce differentiation. This study demonstrates existence "sphingomyelin cycle" Such sphingolipid cycles (Hannun, Y., Bell, R. (1989) Science 243, 500-507) may function signal transduction pathway cellular
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