Cloning and transcriptional activity of ovarian-specific promoter in dairy goats.
作者： Long Bai , Xueqing Liu , Fang Fang , Liang Zhang , HaiBo Zhao
DOI: 10.1016/J.THERIOGENOLOGY.2013.08.007
关键词: DNA binding site 、 Ovarian Cortex 、 Cloning 、 Nucleic acid sequence 、 Stromal cell 、 Biology 、 Oviduct 、 Molecular biology 、 Transcription (biology) 、 Luciferase
摘要: Ovarian-specific promoter (OSP) is a tissue-specific only expressed in the ovary to regulate its development. To investigate activity of OSP diverse dairy goat cells, was cloned from genome and used construct luciferase reporter vector pGL3-OSP examine transcriptional OSP. Consequently, 461-bp fragment obtained. Bioinformatics analysis indicated that contained multiple transcription factor binding sites, such as SRY, HSF, AP-1, C/EBP, CAAT, TATA, so on, nucleotide sequence shared 99% 97% similarity with those OSP-1 OSP-2 rat. In addition, two variants were identified (g.164T deletion g.375T
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