Ovarian epithelial cell lineage-specific gene expression using the promoter of a retrovirus-like element.
作者： Jillian K. Weinstein , Denise C. Connolly , Rudi Bao , Muthu Selvakumaran , Anne P. G. Crijns
DOI:
关键词:
摘要: We have isolated 462 bp of sequence termed ovarian-specific promoter 1 (OSP-1) that is part a retrovirus-like element specifically expressed in the rat ovary. evaluated ability OSP-1 to activate gene expression normal and neoplastic cell lines derived from ovaries rats women. found there was marked specificity to drive reporter expression in an ovarian epithelial lineage manner. The herpes simplex virus thymidine kinase (HSV-TK) under control was sufficiently cancer line specific render ganciclovir∼ 50-fold more toxic A2780 human line compared with clones HCT-116 HT-29 colon lines. Furthermore, ganciclovir had marked antitumor efficacy in vivo severe combined immunodeficient mice bearing A2780OSP-1-HSV-TK as s.c. xenograft. suggest that these data support use tool provide specificity to the therapy ovarian-specific oncogene for creation transgenic mouse models of ovarian cancer.
aacrjournals.org参考文章(18)
Robert F. Ozols, Chemotherapy of ovarian cancer. Seminars in Oncology. ,vol. 18, pp. 219- 234 ,(1998) , 10.1007/978-1-4615-5447-9_7
S. C. Mok, M. I. Edelson, T. C. Hamilton, N. Auersperg, S. W. Johnson, The biology of ovarian cancer Seminars in Oncology. ,vol. 25, pp. 281- 304 ,(1998)
Peter J. O'Dwyer, Andrew K. Godwin, Robert F. Ozols, Kang-Shen Yao, Thomas C. Hamilton, Steven W. Johnson, Evidence for Altered Regulation of γ-Glutamylcysteine Synthetase Gene Expression among Cisplatin-sensitive and Cisplatin-resistant Human Ovarian Cancer Cell Lines Cancer Research. ,vol. 55, pp. 4367- 4374 ,(1995)
Joseph R. Testa, Andrew K. Godwin, David C. Schultz, Takahiro Taguchi, Paul D. Miller, Abbas Abdollahi, Lori A. Getts, Thomas C. Hamilton, Identification of a gene containing zinc-finger motifs based on lost expression in malignantly transformed rat ovarian surface epithelial cells. Cancer Research. ,vol. 57, pp. 2029- 2034 ,(1997)
Alain Gouyette, Olivier Rixe, François Besnard, Michael Brenner, Didier Vandier, Selective Killing of Glioma Cell Lines Using an Astrocyte-specific Expression of the Herpes Simplex Virus-Thymidine Kinase Gene Cancer Research. ,vol. 58, pp. 4577- 4580 ,(1998)
Vera Braiden, Yuji Nagayama, Makoto Iitaka, Hiroyuki Namba, Masami Niwa, Shunichi Yamashita, Retrovirus-Mediated Suicide Gene Prodrug Therapy Targeting Thyroid Carcinoma Using a Thyroid-Specific Promoter Endocrinology. ,vol. 139, pp. 3996- 3999 ,(1998) , 10.1210/ENDO.139.9.6286
A K Godwin, P D Miller, L A Getts, K Jackson, G Sonoda, K J Schray, J R Testa, T C Hamilton, Retroviral-like sequences specifically expressed in the rat ovary detect genetic differences between normal and transformed rat ovarian surface epithelial cells. Endocrinology. ,vol. 136, pp. 4640- 4649 ,(1995) , 10.1210/ENDO.136.10.7664684
Selvarangan Ponnazhagan, Pinku Mukherjee, Mervin C. Yoder, Xu-Shan Wang, Shang Zhen Zhou, Johanne Kaplan, Samuel Wadsworth, Arun Srivastava, Adeno-associated virus 2-mediated gene transfer in vivo: organ-tropism and expression of transduced sequences in mice Gene. ,vol. 190, pp. 203- 210 ,(1997) , 10.1016/S0378-1119(96)00576-8
Eran Silverman, Sarah Eimerl, Joseph Orly, CCAAT Enhancer-binding Protein β and GATA-4 Binding Regions within the Promoter of the Steroidogenic Acute Regulatory Protein (StAR) Gene Are Required for Transcription in Rat Ovarian Cells Journal of Biological Chemistry. ,vol. 274, pp. 17987- 17996 ,(1999) , 10.1074/JBC.274.25.17987
R. J. Schilder, L. Hall, A. Monks, L. M. Handel, A. J. Fornace, R. F. Ozols, A. T. Fojo, T. C. Hamilton, Metallothionein gene expression and resistance to cisplatin in human ovarian cancer. International Journal of Cancer. ,vol. 45, pp. 416- 422 ,(1990) , 10.1002/IJC.2910450306