Electrochemical Quantitation of DNA Immobilized on Gold

摘要: We have developed an electrochemical method to quantify the surface density of DNA immobilized on gold. The DNA, more specifically number nucleotide phosphate residues, is calculated from amount cationic redox marker measured at electrode surface. was gold by forming mixed monolayers thiol-derivitized, single-stranded oligonucleotide and 6-mercapto-1-hexanol. saturated charge-compensating in monolayer, determined using chronocoulometry, directly proportional residues thereby DNA. This permits quantitative determination both single- double-stranded electrodes. Surface densities were precisely varied range (1−10) × 1012 molecules/cm2, as method, monolayers. hybridization efficiency complementary strands a...