Ultraviolet radiation induces release of MIA: a new mechanism for UVR-induced progression of melanoma.

摘要: MIA is a potent melanoma detachment factor that interferes with cellular adherence by binding to fibronectin and laminin, blocking their interaction alpha4beta1 alpha5beta1 integrins. The direct correlation between serum levels of patients progressing melanomas tumor load supports role for as progression factor. goal this study was determine the effect ultraviolet radiation (UVR), activating ras mutation, or loss p53 function on expression release from cells. We previously showed transfection mutant constitutively active into cell line, WM35, induces phenotypic change radial vertical growth, exhibiting increased proliferation migration. Here, we report elevated in ras-transfected line. In addition, functional p53, using dominant negative construct, substantially lowered level compared control. UVR stimulated extracellular compartment both control lines. mRNA following all lines tested. By inducing either apoptosis necrosis, were able confirm protein not released cells due death alone. have identified transcriptional shown dependent upon p53. propose UVR-induced production may promote growth phase basement membrane matrix proteins, serving unique mechanism melanoma.