Evaluation of UV radiation-induced toxicity and biophysical changes in various skin cells with photo-shielding molecules.

摘要: Ultraviolet radiation (UVR) triggers many complex events in different types of skin cells, including benign, malignant and normal cells. Chromophores present these cells play a crucial role various cellular processes. Unprecedented methods are required for the real-time monitoring changes an vitro model exposed to intermittent mild intense UVR determine mechanisms underlying cell degeneration effects unexpected toxic, agonist antagonist agents. This study reports analytical application whole cell-based sensor platform examining biophysical UVR. We used human keratinocyte, melanocyte fibroblast lines normal, pathological protective roles In addition, we examined morphological, biomechanical associated with induced by at 254 365 nm. Information on UVR-induced cytoskeleton ultrastructure, integrity, spreading area, actin microfilament distribution inflammation, microtubule damage, membrane rupture death was characterized loss or increase properties All nm showed significant surface roughness stiffness time-dependent manner. toxicity differently pigmented compared that pretreated melanin, keratin basic growth factor analyze shielding efficiency Melanin exerted effect other two The information obtained this could advance our understanding process, help developing new interventions strategies.