Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells.

摘要: Using a series of techniques to identify and deplete various peripheral blood lymphocyte subpopulations, we studied the cytotoxic reactivity normal individuals against myeloid cell line K-562 in 4-hr 51chromium-release assay. Depletion lymphocytes bearing complement receptors had variable, usually negligible effect on cytotoxicity. In contrast, depletion Fc abrogated target lysis. Separation with high-affinity binding sheep red cells (SRBC) evidenced by rosette formation at 29 degrees C yielded population rosette-forming containing few cells, whereas separation total E-RFC under optimal rosetting conditions produced fraction major proportion effector cells. These data indicate that this system is receptor positive, largely negative, may possess low density or affinity for SRBC.