Completion of the DNA sequence of mouse adenovirus type 1: sequence of E2B, L1, and L2 (18-51 map units).

摘要: Abstract The DNA sequence of 9991 nt, corresponding to 18–51 map units mouse adenovirus type 1 (MAV-1), was determined, completing the Larsen strain MAV-1. length complete MAV-1 genome is 30 946 nucleotides, consistent with previous experimental estimates. unit region encodes early 2B proteins necessary for adenoviral replication as well late L1 and L2 structural packaging proteins. Sequence comparison in this human adenoviruses indicates broad similarities, including colinear preservation all recognized open reading frames (ORFs), highest amino acid identity occurring polymerase polypeptide III (penton base subunit) ORFs. Virus-assciated (VA) RNA not encoded where VA RNAs are found adenoviruses, between E2B L1, nor it anywhere entire genome. lacks arginin-glycine-aspartic (RGD) motif which involved an association cell-surface integrins. Only one RGD identified coding Similar porcine adenovirus, C-terminus fiber protein.