Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues
作者： Heli Nygren , Tuulikki Seppänen-Laakso , Sandra Castillo , Tuulia Hyötyläinen , Matej Orešič
DOI: 10.1007/978-1-61737-985-7_15
关键词: Liquid chromatography–mass spectrometry 、 Mass spectrometry 、 Sample preparation 、 Repeatability 、 Chemistry 、 Chromatography 、 Analytic Sample Preparation Methods 、 Lipidomics
摘要: In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, analysis carried out with ultrahigh-performance liquid chromatography combined mass spectrometry (UPLC-MS). For further identification, MS(n) utilizing an LTQ-Orbitrap as detector. Such system affords determination accurate masses thus highly useful tool lipid identification. repeatability proved to be good, relative standard errors spiked being between 4.51 10.44%. throughput methodology described here over 100 day.
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