Characterizing the mechanism for ginsenoside-induced cytotoxicity in cultured leukemia (THP-1) cells.

摘要: Pure ginsenoside standards (saponins Rh2, PD, and PT), along with an Rh2-enhanced North American ginseng (Panax quinquefolius) leaf extract (LFRh2), were tested for cytotoxic activity in cultured THP-1 leukemia cells. Thermal treatment of resulted production both Rh2 Rg3 content that was confirmed by liquid chromatography – mass spectrometry (LC-MS). Flow cytometry cells stained annexin V – fluorescein isothiocyanate propidium iodide showed the LFRh2 significantly (p ≤ 0.05) increased apoptosis (18% ± 0.4%) after 23 h at a concentration inhibited cell viability 50% (LC50 (72 h) = 52 μg/mL. In comparison, similar significant increase apoptotic numbers occurred 41 h exposure pure standards, PD 13 μg/mL), PT 19 μg/mL), 15 μg/mL). Although no further observed to increasing concentrations PD...