Diol- and triol-type ginseng saponins potentiate the apoptosis of NIH3T3 cells exposed to methyl methanesulfonate
作者： Sung Jin Hwang , Jae Young Cha , Seh Geun Park , Gi Jung Joe , Hyung Min Kim
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摘要: In this study we investigated the effect of ginseng saponins on p53-dependent apoptosis in NIH3T3 cells exposed to methyl methanesulfonate (MMS), an alkylating agent. Trypan blue exclusion assay, cell morphology studies, and apoptotic index determined by acridine orange staining showed that postincubation MMS-exposed medium containing diol- (PD) or triol-type (PT) potentiate death. FACS analysis indicated increased population saponin-postincubation group was accompanied accumulation G0/G1 phase. By Western blot analyses it demonstrated increases expression p53 p21 but decreased CDK2, cyclin E D1, PCNA. The upregulation downregulation CDK2 shown be experiments using antisense oligonucleotide. These results suggest contain components potentiating via activation, with cycle-related protein expression.
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