Glucocorticoid receptor-induced down-regulation of MMP-9 by ginseng components, PD and PT contributes to inhibition of the invasive capacity of HT1080 human fibrosarcoma cells.

摘要: We examined the effects of purified ginseng components, panaxadiol (PD) and panaxatriol (PT), on expression matrix metalloproteinase-9 (MMP-9) in highly metastatic HT1080 human fibrosarcoma cell line. A significant down-regulation MMP-9 by PD PT was detected Northern blot analysis. However, MMP-2 not changed treatment with PT. Quantitative gelatin based zymography confirmed a markedly reduced MMP-9, but To investigate whether level affects invasive capacity cells, we conducted an vitro invasion assay treated cells. The results revealed that tumor through reconstituted basement membrane transwell chamber. Because similarity chemical structure between PD, dexamethasone (Dexa), synthetic glucocorticoid, investigated were mediated nuclear translocation glucocorticoid receptor (GR). Increased GR nucleus cells immunocytochemistry. Western gel retardation assays increase after These suggest GR-induced contributes to reduce