Airway secretory micrornaome changes during rhinovirus infection in early childhood

摘要: Background Innate immune responses are fine-tuned by small noncoding RNA molecules termed microRNAs (miRs) that modify gene expression in response to the environment. During acute infections, miRs can be secreted extracellular vesicles (EV) facilitate cell-to-cell genetic communication. The purpose of this study was characterize baseline population EVs airways young children (airway secretory microRNAome) and examine changes during rhinovirus (RV) infection, most common cause asthma exacerbations important early risk factor for development beyond childhood. Methods Nasal airway secretions were obtained from (≤3 yrs. old) PCR-confirmed RV infections (n = 10) age-matched controls 10). Nasal isolated with polymer-based precipitation global miR profiles generated using NanoString microarrays. We validated our vivo data an vitro epithelium model apical primary human bronchial epithelial cells (HBEC) differentiated at air-liquid interface (ALI). Bioinformatics tools used determine unified (nasal bronchial) signature miRNAome infection children. Results Multiscale analysis identified four comprising miRNAome: hsa-miR-630, hsa-miR-302d-3p, hsa- miR-320e, hsa-miR-612. hsa-miR-155 as main change children. investigated potential biological relevance secretion hsa-mir-155 silico models derived datasets experimental infection. These analyses confirmed targetome is overrepresented pathway upper individuals infected RV. Conclusions Comparative microRNAome indicates associated containing miR-155, which predicted regulate antiviral immunity. Further characterization health disease may lead completely new strategies treat monitor respiratory conditions all ages.