Physical characterization and profiling of airway epithelial derived exosomes using light scattering.

摘要: Exosomes and other extracellular vesicles have been gaining interest during the last decade due to their emerging role in biology and, disease pathogenesis biomarker potential. Almost all published research related exosomes include some form of physical characterization. Therefore, these should be precisely profiled characterized physically before studying biological as intercellular messengers, biomarkers or therapeutic tools. Using a combination light scattering techniques, including dynamic (DLS) multi-angle laser combined with size exclusion separation (SEC-MALLS), we compared distinct derived from apical secretions two different cultured airway epithelial cells. The results indicated that cells release properties sizes. Human primary tracheobronchial cell culture (HTBE) hydrodynamic radius (Rh) approximately 340 nm while gyration (Rg) is 200 nm. Electron microscopy analysis, however, revealed spherical component 40-100 size, they carry filamentous, entangled membrane mucins on surface increases overall radius. mucin decoration defines charge measured using techniques. Their mirror which are derived. This may provide unique tool for researchers elucidate unanswered questions normal innate adaptive defense, remodeling airways inflammation, tumorigenesis metastasis.