Mesenchymal Stem Cell-Specific and Preosteoblast-Specific Ablation of TSC1 in Mice Lead to Severe and Slight Spinal Dysplasia, Respectively.
作者： Cheng Yang , Jianwen Liao , Pinglin Lai , Hai Huang , Shicai Fan
DOI: 10.1155/2020/4572687
关键词: Intramembranous ossification 、 Spinal dysplasia 、 Endochondral ossification 、 Pathology 、 Somatic cell 、 Genetically modified mouse 、 TSC1 、 Biology 、 Mesenchymal stem cell 、 Dysplasia
摘要: Background TSC1-related signaling plays a pivotal role in intramembranous and endochondral ossification processes during skeletogenesis. This study was aimed at determining the significance of TSC1 gene different stages spinal development. Materials Methods. TSC1-floxed mice (TSC1flox/flox) were crossed with Prrx1-Cre or BGLAP-Cre transgenic mesenchymal stem cell- osteoblast-specific TSC1-deficient mice, respectively. Somatic vertebral differences between WT Prrx1-TSC1 null examined 4 weeks after birth. Results No apparent body size abnormalities newborn 4-week- to 2-month-old driver-depleted TSC1. Vertebral intervertebral discs displayed strong dysplasia mice. In contrast, vertebrae only slightly affected, from skeletal preparations no changes BGLAP-TSC1 Conclusion Our data suggest that is crucial for postnatal spine development but discriminative roles stages. Mesenchymal cell-specific ablation led severe early while deletion affected had detectable effects on discs.
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