Molecular cloning of a novel heat induced/chilling tolerance related cDNA in tomato fruit by use of mRNA differential display.
作者： Dina K. Kadyrzhanova , Konstantinos E. Vlachonasios , Philippos Ververidis , David R. Dilley
关键词: Ripening 、 Complementary DNA 、 Lycopersicon 、 Molecular biology 、 cDNA library 、 Biology 、 Gene family 、 Gene 、 Heat shock protein 、 Northern blot
摘要: Chilling injury was circumvented by heat-treating mature green tomatoes (Lycopersicon esculentum, cv. Mountain Springs) at 42 °C for two days prior to storing them 2 one or weeks, whereas fruits stored without preheating developed typical chilling symptoms and failed ripen 20 °C. Using mRNA differential display screening of the cDNA libraries, we have cloned from tomato fruit a full-length HCT1 (heat induced/chilling tolerance related). The protein (17.6 kDa) predicted coding region has high identity with class II cytosolic small HSPs. gene corresponding termed as LeHSP 17.6. Southern-blot hybridization indicates that 17.6 belongs two-member family. Northern blot analysis heat-induced transcript remains up-regulated during subsequent exposure temperatures least week upon transfer ripening day. Fruits which were only chilled show low level expression transcript. We hypothesize may be involved in protecting cell metabolic dysfunctions leading failure caused injury. This is first report smHSPs encoding tomato.
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