Pattern generation with synthetic sensing systems in lipid bilayer membranes

摘要: The objective of this study was to introduce differential sensing techniques synthetic systems that act, like olfactory receptors, as transporters in lipid bilayer membranes. Routine with most alternative chemosensing ensembles, pattern generation has, quite ironically, remained inaccessible bilayers because the number available crossresponsive sensor components has been insufficient. To address challenge, we here report on use cationic hydrazides can react situ hydrophobic analytes produce amphiphiles which turn act countercation activators for polyanionic fluorogenic vesicles. expand dimension signals generated by system, a small collection peptides containing positive charge (guanidinium, ammonium) and one three reactive are prepared. Odorants used examples analytes, perfumes probe compatibility complex matrices, counterion-activated calf-thymus DNA representative polyion–counterion transport system. Principal component hierarchical cluster analysis obtained multidimensional patterns shown differentiate at least 21 single score plot, discriminating also closely related structures such enantiomers, cis–trans isomers, single-atom homologs, well all tested perfumes. Inverse detection provides access acetone. general nature introduced methodology promises find diverse applications current topics biomembrane research.