Histopathological analysis of tilapia gills (Oreochromis niloticus Linnaeus, 1758) exposed to sugarcane vinasse

作者: J.E. Correia , C.A. Christofoletti , A.C.C. Marcato , J.F.U. Marinho , C.S. Fontanetti

DOI: 10.1016/J.ECOENV.2016.10.004

关键词: Animal scienceBioassayAquatic toxicologyTilapiaSerial dilutionOreochromisBiochemical oxygen demandVinasseBiotechnologyBiologyDilution

摘要: Abstract Sugarcane vinasse is one of the main residues generated by transformation cane into ethanol. Because high organic content (COD), biochemical oxygen demand (BOD), low pH, large amount that this residue (15 l for every liter ethanol produced) and their use as fertilizer on sugarcane crop, potentially polluting to soil ecossystem percolation water too. Thus, study aimed assess toxicity analyzing Oreochromis niloticus gills exposed different dilutions (1%, 2.5%, 5% 10%) in two bioassays. The were collected, fixed analyzed using ultra morphological, histological, histochemical techniques. After exposure vinasse, a statistically significant reduction ridges present surface pavimentous cells was observed bioassays; such structures are responsible mucus retention, which helps protect tissue. In addition, an intumescence treatments with well increase chloridric cells. Some striking tissue changes detected epithelial detachment loss integrity secondary lamellae, causing rupture consequent hemorrhage. first bioassay, these at dilution, focus hemorrhage all dilution ratios. second disorganization only 2.5% vinasse. Moreover, both bioassays performed, mucous when compared control. Our results demonstrate toxic action caused histopathological animals four tested. This highlights need caution disposal soil, especially due its capacity being leached or percolated resources, could seriously damage aquatic fauna.

参考文章(36)
Takashi Hibiya, An atlas of fish histology : normal and pathological features Published in <b>1982</b> in Stuttgart by Fischer. ,(1982)
Gary M. Rand, Sam R. Petrocelli, Fundamentals of Aquatic Toxicology ,(1985)
E. Ye &#121 ilada, Genotoxic activity of vinasse and its effect on fecundity and longevity of Drosophila melanogaster. Bulletin of Environmental Contamination and Toxicology. ,vol. 63, pp. 560- 566 ,(1999) , 10.1007/S001289901017
Carla C.C. Cerqueira, Marisa N. Fernandes, Gill Tissue Recovery after Copper Exposure and Blood Parameter Responses in the Tropical Fish Prochilodus scrofa Ecotoxicology and Environmental Safety. ,vol. 52, pp. 83- 91 ,(2002) , 10.1006/EESA.2002.2164
Ömer Faruk Algur, Asim Kadioǧlu, The effects of vinasse on the growth, biomass and primary productivity in pea (Pisum sativum) and sunflower (Helianthus annuus) Agriculture, Ecosystems & Environment. ,vol. 39, pp. 139- 144 ,(1992) , 10.1016/0167-8809(92)90049-H
Ashraf M. Abdel-Moneim, Mohamed A. Al-Kahtani, Omar M. Elmenshawy, Histopathological biomarkers in gills and liver of Oreochromis niloticus from polluted wetland environments, Saudi Arabia Chemosphere. ,vol. 88, pp. 1028- 1035 ,(2012) , 10.1016/J.CHEMOSPHERE.2012.04.001
D H Evans, The fish gill: site of action and model for toxic effects of environmental pollutants. Environmental Health Perspectives. ,vol. 71, pp. 47- 58 ,(1987) , 10.1289/EHP.877147
Cintya Aparecida Christofoletti, Janaína Pedro Escher, Jorge Evangelista Correia, Julia Fernanda Urbano Marinho, Carmem Silvia Fontanetti, Sugarcane vinasse: environmental implications of its use. Waste Management. ,vol. 33, pp. 2752- 2761 ,(2013) , 10.1016/J.WASMAN.2013.09.005
Tatiana da Silva Souza, Fabiana Aparecida Hencklein, Dejanira de Franceschi de Angelis, Carmem Silvia Fontanetti, Clastogenicity of landfarming soil treated with sugar cane vinasse Environmental Monitoring and Assessment. ,vol. 185, pp. 1627- 1636 ,(2013) , 10.1007/S10661-012-2656-3
M.M. Vega, C. Fernández, T. Blázquez, J.V. Tarazona, A. Castaño, Biological and chemical tools in the toxicological risk assessment of Jarama River, Madrid, Spain. Environmental Pollution. ,vol. 93, pp. 135- 139 ,(1996) , 10.1016/0269-7491(96)00034-6