Refolding of Laccase in Dilution Additive Mode with Copper-Based Ionic Liquid

摘要: Ionic liquids (ILs) are molten salts which do not crystallize at room temperature. Tunable physicochemical properties of ILs including hydrophobicity and polarity facilitate their applications in many biological processes. In this study, a copper-based IL was employed order to enhance the refolding efficiency laccase from Trametes versicolor requires copper as cofactor. When 1-ethyl-3-methylimidazolium trichlorocuprate ([EMIM][CuCl3]) added buffer instead urea, yield improved more than 2.7 times compared conventional contains urea. carried out different temperatures (4, 25, 37 °C), highest obtained 25 °C. At low temperature, two conflicting effects, i.e., suppression aggregate formation decrease folding rate, influence protein refolding. contrast, did lysozyme, non-copper-containing protein. From these results, we can conclude that IL, [EMIM][CuCl3], exclusively effective on process copper-containing