The Rate of Synthesis of Glycosaminoglycans and Collagen by Fibroblasts Cultured from Adult Human Liver Biopsies

摘要: Abstract Adult human liver biopsies were cultured from normal, alcoholic hepatitis, chronic active fibrosis plus hepatitis (active cirrhosis), inactive cirrhosis, and drug hepatitis. The synthesis of collagen was estimated in cultures 58 livers by measuring the conversion [14C]proline to [14C]hydroxyproline collagen; that glycosaminoglycans 57 incorporation [3H]acetate 35SO4 into (GAG). procollagen increased only both pulse medium (P < 0.05) chase 0.02). insoluble (active) 0.01), cirrhosis) 0.001), cirrhosis 0.05). Essentially all radioactive GAG soluble culture media. predominant chondroitin-4 or -6-SO4. 0.01) 0.001). The data indicate absence immuno-competent cells their secretory products, tissue showing biopsy evidence vivo may demonstrate vitro. Increased (soluble) not associated with histologically demonstrable overt hepatic vivo, nor it No significant difference paired which contained either 300 mg/dl ethanol 3.75 methylprednisolone compared respective controls.