Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes.
作者： Barbara Azevedo Pereira , Marcio Gilberto Zangeronimo , Miriam Castillo-Martín , Beatrice Gadani , Bruna Resende Chaves
关键词: Superoxide dismutase 、 Andrology 、 Hsp70 、 Blastocyst 、 Chemistry 、 Oocyte 、 In vitro maturation 、 GPX1 、 Cryopreservation 、 Glutathione
摘要: The present study sought to determine whether in vitro maturation (IVM) of pig oocytes a medium supplemented with insulin growth factor-I (IGF-I) and subsequent vitrification or without reduced glutathione (GSH) affect their quality developmental competence, the expression genes involved antioxidant, apoptotic stress responses. In Experiment 1, cumulus-oocyte complexes were matured absence presence IGF-I (100 ng·mL-1) then vitrified-warmed 2 mM GSH. Maturation rate was evaluated before vitrification, oocyte viability, DNA fragmentation relative transcript abundances BCL-2-associated X protein (BAX), BCL2-like1 (BCL2L1), heat shock 70 (HSPA1A), peroxidase 1 (GPX1) superoxide dismutase (SOD1) assessed fresh oocytes. 2, fertilized competence determined. Whereas addition had no effect on maturation, it caused an increase survival This accompanied by concomitant augment abundance HSPA1A decrease BAX. Furthermore, GSH vitrification-warming media increased rates at post-warming. Likewise, action GPX1 BAX transcript. Blastocyst did not differ from counterparts when combined. conclusion, supplementing 100 ng·mL-1 solutions improves cryotolerance IVM oocytes, through mechanism that involves BAX, expression.
frontiersin.org
sci-hub.se 参考文章(54)
Alper Kocyigit, Mesut Cevik, Effects of leukemia inhibitory factor and insulin-like growth factor-I on the cell allocation and cryotolerance of bovine blastocysts. Cryobiology. ,vol. 71, pp. 64- 69 ,(2015) , 10.1016/J.CRYOBIOL.2015.05.068
Jianjun Dai, Caifeng Wu, Caroline W. Muneri, Yingfang Niu, Shushan Zhang, Rong Rui, Defu Zhang, Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability. Cryobiology. ,vol. 71, pp. 291- 298 ,(2015) , 10.1016/J.CRYOBIOL.2015.08.002
D Rath, C R Long, J R Dobrinsky, G R Welch, L L Schreier, L A Johnson, In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer. Journal of Animal Science. ,vol. 77, pp. 3346- 3352 ,(1999) , 10.2527/1999.77123346X
Lin Xie, Qiongyan Tang, Ling Yang, Lianyi Chen, Insulin-like growth factor I promotes oocyte maturation through increasing the expression and phosphorylation of epidermal growth factor receptor in the zebrafish ovary. Molecular and Cellular Endocrinology. ,vol. 419, pp. 198- 207 ,(2016) , 10.1016/J.MCE.2015.10.018
Yangyang Pan, Yan Cui, Honghong He, Abdul Rasheed Baloch, Jiangfeng Fan, Gengquan Xu, Junfeng He, Kun Yang, Guyue Li, Sijiu Yu, None, Developmental competence of mature yak vitrified-warmed oocytes is enhanced by IGF-I via modulation of CIRP during in vitro maturation. Cryobiology. ,vol. 71, pp. 493- 498 ,(2015) , 10.1016/J.CRYOBIOL.2015.10.150
Shiyong Li, Hetao Bian, Zhe Liu, Ye Wang, Jianghua Dai, Wenfeng He, Xingen Liao, Rongrong Liu, Jun Luo, Chlorogenic acid protects MSCs against oxidative stress by altering FOXO family genes and activating intrinsic pathway. European Journal of Pharmacology. ,vol. 674, pp. 65- 72 ,(2012) , 10.1016/J.EJPHAR.2011.06.033
Marta Wasielak, Takashi Fujii, Tsubasa Ohsaki, Tsutomu Hashizume, Marek Bogacki, Ken Sawai, Transcript abundance and apoptosis in day-7 porcine blastocyst cultured with exogenous insulin-like growth factor-I Reproductive Biology. ,vol. 13, pp. 58- 65 ,(2013) , 10.1016/J.REPBIO.2013.01.173
Chiara Bernardini, Paolo Fantinati, Augusta Zannoni, Monica Forni, Carlo Tamanini, Maria Laura Bacci, Expression of HSP70/HSC70 in swine blastocysts: effects of oxidative and thermal stress. Molecular Reproduction and Development. ,vol. 69, pp. 303- 307 ,(2004) , 10.1002/MRD.20143
Guilherme Oberlender, Luis D.S. Murgas, Márcio G. Zangeronimo, Thais P. Pontelo, Tila A. Menezes, Adriana C. Silva, Concentração do fator de crescimento semelhante à insulina tipo I no fluido folicular suíno coletado a partir de folículos ovarianos de diferentes diâmetros Pesquisa Veterinaria Brasileira. ,vol. 33, pp. 1269- 1274 ,(2013) , 10.1590/S0100-736X2013001000013
S.J. Picco, J.M. Anchordoquy, D.G. de Matos, J.P. Anchordoquy, A. Seoane, G.A. Mattioli, A.L. Errecalde, C.C. Furnus, Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes Theriogenology. ,vol. 74, pp. 1141- 1148 ,(2010) , 10.1016/J.THERIOGENOLOGY.2010.05.015