Cell proliferation analysis is a reliable predictor of lack of carcinogenicity: Case study using the pyrethroid imiprothrin on lung tumorigenesis in mice

摘要: Abstract In the mouse carcinogenicity study, an apparent increase in lung adenocarcinoma was observed male mice at 7000 ppm. Based on overall evaluation of toxicology, oncology, pathology and statistics, we concluded that tumors is not relevant for imiprothrin (Regul Toxicol Pharmacol, 105, 1–14, 2019). To investigate whether has any mitogenic effect Club cells, present study examined its effects replicative DNA synthesis cells histopathology treated with 7 days 3500 7000 ppm diet. Isoniazid, a known mitogen tumor inducer, also 1000 ppm diet as positive control cell mitogenesis morphological changes. Neither nor isoniazid caused necrotic changes by light or electron microscopy. There were no increases bromodeoxyuridine (BrdU) labeling index groups, while there statistically significant BrdU group. These findings demonstrate does induce proliferation morphologic changes, supporting our previous conclusion described above. Thus, should be classified carcinogen. Furthermore, this indicates short-term studies focusing can reliable predicting lack carcinogenic potential test chemicals.