Inhibition of HIV-1 replication and NF-kappa B activity by cysteine and cysteine derivatives.

摘要: HIV-1 proviral DNA contains two binding sites for the transcription factor NF-kappa B. HIV-1-infected individuals have, on average, abnormally high levels of tumour necrosis alpha (TNF alpha) and low plasma cysteine levels. We therefore investigated effects related thiols replication B expression. The experiments in this report show that or N-acetylcysteine (NAC) raise intracellular glutathione (GSH) level inhibit persistently infected Molt-4 U937 cells. However, inhibition appears not to be directly correlated with GSH Cysteine NAC also activity as determined by electrophoretic mobility shift assays chloramphenicol acetyl-transferase (CAT) gene expression under control uninfected This suggests deficiency may cause an over-expression B-dependent genes enhance replication. considered treatment individuals.