Influence of redox status of lymphocytes and monocytes on HIV expression and immune functions. Evaluation in vitro of antioxidant molecules as potential anti-HIV therapy

摘要: We used BHA, a phenolic, lipid-soluble, chain-breaking antioxidant to show that peroxyl radical scavenging blocks NF-1(B activation and HIV1 enhancer activity in PMA- or TNF-stimulated lymphoblastoid T (J.Jhan) monocytic (U937) cell lines. The anti-oxidative effect of BHA was accompanied by an increase thiol, but not glutathione, content stimulated unstimulated cells, whereas TNF stimulation itself barely modified the cellular thiol level. Oxidative stress obtained addition H2O2 culture medium JJhan U937 cells could induce NF-κB activation. These observations suggest PMA do lead through induction changes redox status. Rather, can exert full only if are basal equilibrium tending towards oxidation since prior modification reduction treatment prevents their effects.