Immune oxidative injury induced in mice exposed to normobaric O2: effects of thiol compounds on the splenic cell sulfhydryl content and Con A proliferative response.

摘要: In vivo exposure of mice to normobaric O2 depresses the cellular immune response by a mechanism that remains unknown. vitro oxidative injury leads decreased sulfhydryl groups (SH) in lymphocytes. To determine whether would have similar effects, we measured SH content spleen cells both from had been exposed (O2 SC) and controls ambient air (Air SC). The fresh SC was slightly decreased, whereas after 48 hr culture, proliferative these were found vary with type concentration thiol or disulfide compounds added culture medium. Under standard conditions, i.e., RPMI 1640 medium containing 0.41 mM half-cystine, sharply about 10 20% Air absence presence Con A (2 micrograms/ml), respectively. 20.5% +/- 3.2 SC. cystine-free supplemented various concentrations L-cystine, L-cystine 2-mercaptoethanol (2-ME), L-cysteine, reduced glutathione (GSH), varied parallel correlated (p less than 0.01). Half-cystine (0.41 mM) plus 2-ME (5 X 10(-5) M) L-cysteine alone (4 completely protected induced 82% 6 controls. GSH mM), 79 67.5% SC, Other effective. Oxygen scavengers such as SOD, catalase, mannitol, vitamin E did not protect against decrease damage might be related part membrane lipid peroxidative process. These data show lesions splenic manifested under conditions response. extent alterations could modulated variations environment. Protection protection