Inhibition of murine T-cell responses by anti-oxidants: the targets of lipo-oxygenase pathway inhibitors.

摘要: We have previously established that oxidative phenomena are involved in human T-cell activation (Sekkat, Dornand & Gerber, 1988). In the present work we studied effect of different anti-oxidants (scavengers O2-, .OH and lipo-oxygenase inhibitors) on stimulation murine T cells. report here all used suppressed T-lymphocyte proliferation IL-2 synthesis, former resulting very likely from latter. This inhibition was concomitant with triggering activation. also demonstrate various biochemical targets. Unlike other compounds, phenolic drugs nordihydroguaiaretic acid (NDGA) butylated hydroxyanisole (BHA), which block lipid peroxidation, affect both signals triggered by binding lectin to its receptors: they suppress rise intracellular free calcium concentration inhibit some events, depending sole protein kinase C activation, namely receptor expression phorbol myristate acetate (PMA)-induced pH change. Our results discussed within framework a possible involvement reactive oxygen species arachidonic derivative(s) production.