Single molecule localization deep within thick cells; a novel super-resolution microscope
作者： Reza Tafteh , David R. L. Scriven , Edwin D. W. Moore , Keng C. Chou
关键词: Square (algebra) 、 Position (vector) 、 Filter (signal processing) 、 Microscopy 、 Calibration 、 Microscope 、 Electron tomography 、 Chemistry 、 Optics 、 Time domain 、 Analytical chemistry
摘要: A novel 3D imaging system based on single-molecule localization microscopy is presented to allow high-accuracy drift-free (<0.7 nm lateral; 2.5 axial) many microns deep into a cell. When within the cell, distortions of point-spread function result in an inaccurate and very compressed Z distribution. For accurately represent position each blink, series depth-dependent calibrations are required. The its allied methodology applied image ryanodine receptor cardiac myocyte. Using calibration, receptors cell spread over range that hundreds nanometers greater than implied by conventional analysis. We implemented time domain filter detect overlapping blinks were not filtered stringent goodness fit criterion. This enabled us resolve structure individual (30 square) giving similar obtained with electron tomography.
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