Crystallization and preliminary X‐ray crystallographic studies of RepDC, a hybrid rolling‐circle plasmid replication initiator protein
作者： Dmitry E. Klimenko , Máire A. Convery , Siân Rowsell , Christopher D. Thomas , Simon E. V. Phillips
DOI: 10.1107/S0907444999003005
关键词: Plasmid 、 X-ray 、 Chemistry 、 Crystallization 、 Monomer 、 Crystallography 、 Fusion 、 Rolling circle replication 、 Synchrotron Radiation Source 、 Solvent
摘要: The hybrid plasmid-replication initiator protein RepDC, which is a fusion of the catalytic fragment RepD and DNA-binding RepC from Staphylococcus aureus, has been successfully crystallized X-ray data to 3.5 A have collected on synchrotron radiation source. Crystals belong space group I4132 with unit-cell dimensions = b c 165.1 A. crystals are estimated contain one monomer per asymmetric unit, 55% solvent content.
sci-hub.se 参考文章(16)
A. Rasooly, P.Z. Wang, R.P. Novick, Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer. The EMBO Journal. ,vol. 13, pp. 5245- 5251 ,(1994) , 10.1002/J.1460-2075.1994.TB06856.X
R R Koepsel, R W Murray, W D Rosenblum, S A Khan, Purification of pT181-encoded repC protein required for the initiation of plasmid replication. Journal of Biological Chemistry. ,vol. 260, pp. 8571- 8577 ,(1985) , 10.1016/S0021-9258(17)39511-X
J M Zock, P Birch, S A Khan, Specificity of RepC protein in plasmid pT181 DNA replication. Journal of Biological Chemistry. ,vol. 265, pp. 3484- 3488 ,(1990) , 10.1016/S0021-9258(19)39794-7
C D Thomas, D F Balson, W V Shaw, In vitro studies of the initiation of staphylococcal plasmid replication. Specificity of RepD for its origin (oriD) and characterization of the Rep-ori tyrosyl ester intermediate. Journal of Biological Chemistry. ,vol. 265, pp. 5519- 5530 ,(1990) , 10.1016/S0021-9258(19)39392-5
Christopher D. Thomas, Theo T. Nikiforov, Bernard A. Connolly, William V. Shaw, Determination of Sequence Specificity between a Plasmid Replication Initiator Protein and the Origin of Replication Journal of Molecular Biology. ,vol. 254, pp. 381- 391 ,(1995) , 10.1006/JMBI.1995.0625
Steven J. Projan, John Kornblum, Soraya L. Moghazeh, Irit Edelman, Maria L. Gennaro, Richard P. Novick, Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus. Molecular Genetics and Genomics. ,vol. 199, pp. 452- 464 ,(1985) , 10.1007/BF00330758
R P Novick, Staphylococcal Plasmids and their Replication Annual Review of Microbiology. ,vol. 43, pp. 537- 565 ,(1989) , 10.1146/ANNUREV.MI.43.100189.002541
B.W. Matthews, Solvent content of protein crystals. Journal of Molecular Biology. ,vol. 33, pp. 491- 497 ,(1968) , 10.1016/0022-2836(68)90205-2
Serban Iordanescu, Characterization of the Staphylococcus aureus chromosomal gene pcrA, identified by mutations affecting plasmid pT181 replication. Molecular Genetics and Genomics. ,vol. 241, pp. 185- 192 ,(1993) , 10.1007/BF00280216
Sadhan Majumder, Richard P. Novick, Intermediates in plasmid pT181 DNA replication Nucleic Acids Research. ,vol. 16, pp. 2897- 2912 ,(1988) , 10.1093/NAR/16.7.2897