Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer.

摘要: Abstract The Staphylococcus aureus rolling circle plasmid pT181 regulates its replication by controlling the synthesis of initiator protein RepC. RepC is inactivated during addition an oligodeoxynucleotide, giving rise to a new form, RepC*. We analyzed and RepC* in four classes mutants: copy number mutants, two mutants affecting different portions oriC (origin) mutants. have found that cell with wild-type there are similar relative amounts RepC*, regardless number, conversion dependent. Genetic biochemical evidence presented functions as dimer homodimer converted RepC/RepC* heterodimer.