Cryopreservation and short-term storage of sturgeon sperm, a review
作者： R Billard , J Cosson , S.B Noveiri , M Pourkazemi
DOI: 10.1016/J.AQUACULTURE.2003.10.029
关键词: Sturgeon 、 Human fertilization 、 Cryoprotectant 、 Sperm 、 Animal science 、 Ecology 、 Artificial reproduction 、 Cryopreservation 、 Yolk 、 Milt 、 Biology
摘要: Abstract Sturgeon sperm was cryopreserved in liquid nitrogen or stored at 1–4 °C on a short-term basis. The objective to establish reliable technologies for the conservation of certain endangered sturgeon species and artificial reproduction farms. Cryopreseved diluted buffered extenders (pH between 8 8.5) containing intracellular (dimethyl-sulfoxide (DMSO), methanol, ethylene glycol) extracellular (saccharose, egg yolk) cryoprotectants. concentration cryoprotectants ranged from 5% 20%. Tested freezing rates were range 0.5–18.5 °C/min thawing rate 15 40 5 6 s. Ten eight percent motile after thawing. Fertilization up 73–94% reported thawed but most cases, average values motility non-frozen activated few days vitro storage. Motility declined with time depending oxygen availability milt, temperature (optimum near 0 °C) pH; though some spermatozoa still several weeks
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