The influence of cryoprotectants on sturgeon (Acipenser ruthenus) sperm quality, DNA integrity, antioxidant responses, and resistance to oxidative stress
作者： Anna Shaliutina-Kolešová , Jacky Cosson , Ievgen Lebeda , Ievgenia Gazo , Olena Shaliutina
DOI: 10.1016/J.ANIREPROSCI.2015.05.014
关键词:
摘要: Abstract This study examined the effect of cryoprotectants on DNA integrity, antioxidant defense, and resistance to oxidative stress in cryopreserved sterlet Acipenser ruthenus sperm. The freeze-thaw process significantly influenced sperm motility, with significant differences among cryoprotectants. In vitro exposure xanthine–xanthine oxidase (X–XO) system as a model reactive oxygen species inducer resulted lesser motility rate velocity compared control, there was decrease these variables time- dose-dependent manner. greatest X (0.6 mM)–XO (0.05 U/mL) concentration incubation period (30 min) associated 62% fragmentation 10% ethylene glycol (EG). maximum lipid peroxidation (LPO) carbonyl derivatives proteins (CP) also observed EG exposed X–XO at 0.6 mM X–0.05 U/mL XO. frozen/thawed containing that dimethyl sulfoxide (DMSO) had enhancement superoxide dismutase (SOD) glutathione reductase (GR) activity. current confirms is not effective for cryopreservation, were highly sensitive free radicals after cryopreservation EG.
cabdirect.org
elsevier.com
researchgate.net 
sci-hub.se 参考文章(38)
J. Stoss, W. Holtz, Cryopreservation of rainbow trout (Salmo gairdneri) sperm: IV. The effect of DMSO concentration and equilibration time on sperm survival, sucrose and KCl as extender components and the osmolality of the thawing solution Aquaculture. ,vol. 32, pp. 321- 330 ,(1983) , 10.1016/0044-8486(83)90229-6
I Carlberg, B Mannervik, Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. ,vol. 250, pp. 5475- 5480 ,(1975) , 10.1016/S0021-9258(19)41206-4
S. Boryshpolets, B. Dzyuba, M. Rodina, S. M. H. Alavi, D. Gela, O. Linhart, Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants Journal of Applied Ichthyology. ,vol. 27, pp. 1147- 1149 ,(2011) , 10.1111/J.1439-0426.2011.01866.X
Kenneth B. Storey, Oxidative stress: animal adaptations in nature Brazilian Journal of Medical and Biological Research. ,vol. 29, pp. 1715- 1733 ,(1996)
E. Cabrita, C. Sarasquete, S. Martínez-Páramo, V. Robles, J. Beirão, S. Pérez-Cerezales, M.P. Herráez, Cryopreservation of fish sperm: applications and perspectives Journal of Applied Ichthyology. ,vol. 26, pp. 623- 635 ,(2010) , 10.1111/J.1439-0426.2010.01556.X
A. Ciereszko, G.P. Toth, S.A. Christ, K. Dabrowski, Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa. Theriogenology. ,vol. 45, pp. 665- 672 ,(1996) , 10.1016/0093-691X(95)00412-2
Ievgeniia Gazo, Anna Shaliutina-Kolešová, Mariola A. Dietrich, Pavla Linhartová, Olena Shaliutina, Jacky Cosson, The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpio L.) spermatozoa Molecular Reproduction and Development. ,vol. 82, pp. 48- 57 ,(2015) , 10.1002/MRD.22442
R Billard, J Cosson, S.B Noveiri, M Pourkazemi, Cryopreservation and short-term storage of sturgeon sperm, a review Aquaculture. ,vol. 236, pp. 1- 9 ,(2004) , 10.1016/J.AQUACULTURE.2003.10.029
R. Billard, Effects of coelomic and seminal fluids and various saline diluents on the fertilizing ability of spermatozoa in the rainbow trout, Salmo gairdneri. Reproduction. ,vol. 68, pp. 77- 84 ,(1983) , 10.1530/JRF.0.0680077
Gyung Ja Choi, Hee Jae Lee, Kwang Yun Cho, Lipid Peroxidation and Membrane Disruption by Vinclozolin in Dicarboximide-Susceptible and -Resistant Isolates ofBotrytis cinerea Pesticide Biochemistry and Physiology. ,vol. 55, pp. 29- 39 ,(1996) , 10.1006/PEST.1996.0032