Molecular cloning of the chicken myelomonocytic growth factor (cMGF) reveals relationship to interleukin 6 and granulocyte colony stimulating factor

摘要: Normal as well retrovirally transformed avian myeloid precursor cells require the colony stimulating factor cMGF for their survival, proliferation and formation in vitro. has been shown to be a glycoprotein which is active picomolar concentration range. Co-expression of kinase type oncogenes v-myb or v-myc induces expression confers independence via an autocrine mechanism. Here we describe molecular cloning from myeloblast cDNA library show that it 201 amino acid residue secretory protein modified by signal peptide cleavage glycosylation during translocation into lumen membrane vesicles. A bacterially expressed trpE-cMGF fusion E26 myeloblasts bioassay suggesting not absolutely necessary biological activity. Sequence comparison reveals distantly related G-CSF IL-6.