Sequence of cDNAs Encoding Components of Vascular Actin Single-stranded DNA-binding Factor 2 Establish Identity to Purα and Purβ
作者： Robert J. Kelm , Paula K. Elder , Arthur R. Strauch , Michael J. Getz
关键词: Enhancer 、 Transcription (biology) 、 Biology 、 DNA replication 、 Transcription factor 、 DNA-binding protein 、 cDNA library 、 Complementary DNA 、 Molecular biology 、 Gene
摘要: Transcriptional repression of the mouse vascular smooth muscle alpha-actin gene in fibroblasts and myoblasts is mediated, part, by interaction two single-stranded DNA binding activities with opposite strands an essential transcription enhancer factor-1 recognition element (Sun, S., Stoflet, E. Cogan, J. G., Strauch, A. R., Getz, M. (1995) Mol. Cell. Biol. 15, 2429-2436). One these activities, previously designated actin DNA-binding factor 2 includes distinct polypeptides (p44 p46) which specifically interact purine-rich strand both a related protein coding exon (Kelm, R. J., Jr., Sun, (1996) Chem. 271, 24278-24285). Expression screening lung cDNA library has now resulted isolation clones that encode p46 p44. proteins identical to Puralpha, retinoblastoma-binding implicated transcriptional activation replication. The other family member, presumably Purbeta. Comparative band shift Southwestern blot analyses conducted cellular p46, p44, cloned Pur synthesized vitro vivo, establish identity Puralpha p44 This study implicates and/or Purbeta control transcription.
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