The murine decorin. Complete cDNA cloning, genomic organization, chromosomal assignment, and expression during organogenesis and tissue differentiation.

摘要: Decorin, a proteoglycan known to interact with collagen and growth factors, may play key roles during ontogenesis, tissue remodeling, cancer. We have deciphered the complete protein sequence of murine decorin by cDNA cloning, elucidated its gene structure chromosomal location, investigated expression in developing embryo. The were highly conserved vis human counterpart; however, lacked leader exon, exon Ib, which was found only human. Using interspecific backcrossing, we assigned chromosome 10 just proximally Steel locus. In situ hybridization studies mouse embryos showed distinct pattern progressive increase mRNA ontogenesis. At early stages (day 11 postconception), detectable floor plate region. Subsequently (days 13-16 especially prominent meninges mesothelial linings pericardium, pleura, coelomic cavity, as well dermis subepithelial layers intestine urinary bladder. contrast, major parenchymal organs weakly positive for mRNA. These findings suggest that role epithelial/mesenchymal interactions organ development shaping.