Enumeration and characterization of DJH structures in mouse fetal liver.

摘要: Abstract The primary immunoglobulin (Ig) repertoire in the mouse develops during fetal life liver. The first Ig gene rearrangement--the joining of a DH to JH segment--contributes largely diversity found CDR3, as well potentially encoding D mu protein which is believed function development B cell. In this report, number DJH joins two strains, C57BL/6 and BALB/c, were enumerated from days 12 16 development. It was that structures increased less than 300 per liver on day greater 700,000 (C57BL/6) 300,000 (BALB/c) 16. Each segment used approximately equally each examined. When examined by cloning sequencing it reading frame (RF) usage (with respect JH) not random--RF1 70% time. Moreover, single segment, DFL16.1, 50% all reinforcing notion restricted its antigen binding potential.