Enzymatic alterations in single type IIB skeletal muscle fibers with inactivity and exercise in 12- and 30-month-old rats.
作者： Jon J. Groskreutz , LaDora V. Thompson
DOI: 10.1007/BF03324461
关键词: Gene isoform 、 Skeletal muscle fiber adaptation 、 Analysis of variance 、 Lactate dehydrogenase 、 Anatomy 、 Dehydrogenase 、 Internal medicine 、 Endocrinology 、 Chemistry 、 Hindlimb 、 Enzyme 、 Myosin
摘要: The purpose of the present study was to examine effects aging, inactivity and weight-bearing exercise on fast-twitch single Type IIB skeletal muscle fibers from superficial region lateral head gastrocnemius (Type fibers). Specifically, this compared biochemical properties after 7 days hindlimb unweighting (HU), HU with intermittent (HU-Ex), cage control (C) adult aged Fischer 344 Brown Norway F1 Hybrid rats (12- 30-month old). Biochemical measurements included total lactate dehydrogenase (LDH) β-hydroxy-acyl-coenzyme A activities (BHAD), expressed in nmoles/μg/hr dry weight. Fiber-typing for myosin heavy chain isoform determined by SDS-PAGE. With age, LDH activity decreased 52.0±3.4 (12-month old) 39.5±2.9 (30-month Following HU, increased 22% (52.0±3.4 66.4±3.2 nmoles/μg/hr) 12-month-old animals, whereas no difference observed 30-month-old animals. HU-Ex, animals not significantly different that a significant increase (38.1±2.9 51.8±3.1 respectively. Analysis variance revealed an interaction between age condition, indicating have response inactivity. enzyme BHAD were experimental conditions. results demonstrate decrease suggesting age-related shift profile. Further, fiber adaptation is age-dependent.
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