Hydroperoxide lyase depletion in transgenic potato plants leads to an increase in aphid performance.

摘要: Hydroperoxide lyases (HPLs) catalyze the cleavage of fatty acid hydroperoxides to aldehydes and oxoacids. These volatile play a major role in forming aroma many plant fruits flowers. In addition, they have antimicrobial activity vitro thus are thought be involved defense response against pest pathogen attack. An HPL present potato leaves has been characterized shown cleave specifically 13-hydroperoxides both linoleic linolenic acids yield hexanal 3-hexenal, respectively, 12-oxo-dodecenoic acid. A cDNA encoding this isolated used monitor gene expression healthy mechanically damaged plants. is subject developmental control, being high young attenuated older ones, it induced weakly by wounding. enzymatic activity, nevertheless, remains constant different ages also after wounding, suggesting that posttranscriptional mechanisms may regulate its levels. Antisense-mediated depletion transgenic plants identified enzyme as route 13-fatty hydroperoxide degradation leaves. Although these highly reduced levels show no phenotypic differences compared with wild-type particularly regard wound-induced genes. However, aphids feeding on HPL-depleted display approximately two-fold increase fecundity above those nontransformed plants, consistent hypothesis HPL-derived products negative impact aphid performance. Thus, HPL-catalyzed production C6 key step built-in resistance mechanism some sucking insect pests.