Renewable standard reference material for the detection of TP53 mutations.
作者： Catherine D. O’Connell , Lois A. Tully , Joseph M. Devaney , Michael A. Marino , John P. Jakupciak
DOI: 10.1007/BF03260024
关键词: Plasmid 、 Gene 、 Genetics 、 Biology 、 DNA 、 Exon 、 Amino acid 、 Polymerase chain reaction 、 Mutagenesis (molecular biology technique) 、 Amplicon 、 Molecular biology
摘要: Background: Numerous DNA-based tests are currently in use or under development for the detection of mutations associated with disease. Most current methods PCR amplification technologies and after separation chromatography products. We have developed a panel standard reference materials consisting 12 plasmid clones containing 2.0kb region TP53 gene, including exons 5–9. Eleven these contain single mutation within mutational hot spots twelfth is wild-type this gene. The amino acid (aa) 128: C to T; aa 175: G A; 237: T C; 245: 248: 249: 273: 282: 328: C. These (SRMs), created by site-directed mutagenesis from human cell line, include specific most commonly found be cancer. Their will improve disease serving as validation monitor errors measurement methods, amplification, amplicon separation, data analysis different technology platforms.
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