Cytochrome oxidase and its derivatives V. The reaction of ferrous cytochrome c oxidase with oxygen and hydrogen peroxide in the presence of sodium dithionite

摘要: Summary The findings of Okunuki and Wainio are confirmed that on oxygenation cytochrome oxidase ferrocytochrome c :oxygen oxidoreductase, EC 1.9.3.1) reduced by sodium dithionite a compound with Soret band at about 428 mμ is formed which differs from ferric oxidase, this not mixture ferrous oxidase. product does contain ferrocytochromes or 3 , while it cannot be excluded may ferricytochrome . In contrast to no significant difference has been found between the action hydrogen peroxide dithionite. gradually reverts This reaction accelerated catalase, but ferricyanide. It converted evacuation, nor CO-oxidase carbon monoxide in absence reducing substances. These observations support hypothesis peroxidic, rather than reversible oxygen “cytochrome ” both cytochromes involved its formation. There gome evidence atmospheric due autoxidation Alternative mechanisms discussed, direct interaction labile