Microelectrophoretic method for the detection of proteinase inhibitors
作者： Dinah S. Seidl , Werner G. Jaffé , Eglis González , Antonio Calleias
DOI: 10.1016/0003-2697(78)90439-6
关键词: Casein 、 Biochemistry 、 Electrophoresis 、 Substrate (chemistry) 、 Chromatography 、 Digestion 、 Amido Black 、 Trypsin 、 Subtilisin 、 Filter paper 、 Biology 、 Biophysics 、 Cell biology 、 Molecular biology
摘要: Abstract A microelectrophoretic method for the detection of proteinase inhibitors is deseribed. Microscope slides covered with agar gel containing casein were used electrophoretic separation proteins from bean seeds. Subsequently, a filter paper strip saturated solution known concentration. After 60 min digestion, strips removed, and stained amido black. Blue spots appeared where substrate was protected digestion by presence inhibitors. Single seed or leaf extracts can be studied this method. Different trypsin inhibition patterns observed samples different varieties. An inhibitor subtilisin detected in all Ph. vulgaris studied.
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sci-hub.se 参考文章(14)
Karl A. Wilson, M. Laskowski, Isolation of three isoinhibitors of trypsin from garden bean, Phaseolus vulgaris, having either lysine or arginine at the reactive site. Journal of Biological Chemistry. ,vol. 248, pp. 756- 762 ,(1973) , 10.1016/S0021-9258(19)44332-9
M. Sandholm, R. R. Smith, J. C. H. Shih, M. L. Scott, Determination of antitrypsin activity on agar plates: relationship between antitrypsin and biological value of soybean for trout. Journal of Nutrition. ,vol. 106, pp. 761- 766 ,(1976) , 10.1093/JN/106.6.761
J. Curtis Melville, Clarence A. Ryan, Chymotrypsin Inhibitor I from Potatoes Journal of Biological Chemistry. ,vol. 247, pp. 3445- 3453 ,(1972) , 10.1016/S0021-9258(19)45161-2
William M. Awad, Aida R. Soto, Steven Siegel, William E. Skiba, Gary G. Bernstrom, Maria S. Ochoa, The Proteolytic Enzymes of the K-1 Strain of Streptomyces griseus Obtained from a Commercial Preparation (Pronase) Journal of Biological Chemistry. ,vol. 247, pp. 4144- 4154 ,(1972) , 10.1016/S0021-9258(19)45052-7
Moshe Trop, Yehudith Birk, The specificity of proteinases from Streptomyces griseus (pronase) Biochemical Journal. ,vol. 116, pp. 19- 25 ,(1970) , 10.1042/BJ1160019
JOSÉ URIEL, JOSETTE BERGES, Characterization of Natural Inhibitors of Trypsin and Chymotrypsin by Electrophoresis in Acrylamide-Agarose Gels Nature. ,vol. 218, pp. 578- 580 ,(1968) , 10.1038/218578B0
M. KUNITZ, CRYSTALLIZATION OF A TRYPSIN INHIBITOR FROM SOYBEAN. Science. ,vol. 101, pp. 668- 669 ,(1945) , 10.1126/SCIENCE.101.2635.668
Jurgen Weiel, Kenneth D. Hapner, Barley proteinase inhibitors: A possible role in grasshopper control? Phytochemistry. ,vol. 15, pp. 1885- 1887 ,(1976) , 10.1016/S0031-9422(00)88837-8
N. Heimburger, H. Haupt, Charakterisierung von α1 X-glykoprotein als chymotrypsin-lnhibitor des humanplasmas Clinica Chimica Acta. ,vol. 12, pp. 116- 118 ,(1965) , 10.1016/0009-8981(65)90118-X
Dinah S. Seidl, Mercedes Jaffe, Werner G. Jaffe, Digestibility and proteinase inhibitory action of a kidney bean globulin Journal of Agricultural and Food Chemistry. ,vol. 17, pp. 1318- 1321 ,(1969) , 10.1021/JF60166A004