Isoelectric focusing of subtilisin inhibitors: detection and partial characterization of cereal inhibitors of chymotrypsin and microbial proteases.

摘要: Abstract Inhibitors of subtilisin and other microbial serine proteases were detected after isoelectric focusing cereal extracts in polyacrylamide gels by a negative staining technique based on the chromogenic substrate acetyl- d,l -phenylalanine-2-naphthyl ester. The 10 chymotrypsin isoinhibitors identified experiments with barley also inhibitors subtilisin, sensitivity detection was about 25 times higher when stained for this enzyme. In addition, one specific inhibitor zone detected. These 11 similar wheat, triticale, rye, oats, sorghum, rice, maize further characterized classified focusing. Techniques used purpose included application enzymes different specificity inhibition, comparison varieties, pretreatment extract monospecific antibodies toward purified from same or related species, affinity gel. procedure effective separation sodium dodecyl sulfate-gel electrophoresis.