Isolation of human urinary protease inhibitor by single-step affinity chromatography
作者： A. Kessner , L.T. Hodgins , W. Troll
DOI: 10.1016/0003-2697(79)90675-4
关键词:
摘要: Human urinary protease inhibitor was isolated from freshly collected male urine by a single chromatographic step utilizing bovine α-chymotrypsin (EC 3.4.21.1) covalently bound to cross-linked agarose. The adsorbent prepared new series of chemical reactions using trichloro-s-triazine as coupler. Study parameters, including pH, temperature, and ionic strength, led simplified elution system for removing contaminants. antitryptic-antichymotryptic activity the appeared be unaltered chromatography.
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