[21] Methods for the attachment of haptens and proteins to erythrocytes

摘要: Publisher Summary This chapter discusses the methods for attachment of haptens and proteins to erythrocytes. Erythrocytes which antigens or antibodies are adsorbed conjugated have been used extensively as target cells detection antigenic substances. The reaction antigen-bearing erythrocytes with complementary antibody results in agglutination initiates a complement-dependent hemolysis. quantitation antigen by inhibition passive immune Such indicator also quantitate antigen-binding rosette assay antibody-forming plaque-forming cell assays. Moreover, these can be enumerating reverse counting antigen-secreting assay. Assays using detect hybridomas secreting desired found elsewhere. In addition, new coupling discussed this chapter.