Development of a Hemolytic Plaque Assay for Glutamic Acid, Lysine-Containing Polypeptides: Demonstration that Nonresponder Mice Produce Antibodies to These Peptides When Conjugated to an Immunogenic Carrier

摘要: Fowl γ-globulin, when chemically conjugated to GLO or GL, functions as a T-dependent immunogenic carrier and stimulates anti-GLO anti-GL antibody production in nonresponder mice. The conjugation procedure utilizes the Schiff base reaction. responses were detected by hemagglutination hemolytic plaque assays using GL-coated erythrocytes. coupling of GL erythrocytes novel which palmitoyl derivative is adsorbed onto red blood cells. optimal conditions for preparing SRBC are presented. With assay, we have verified that responder animals make both IgM IgG responses, whereas mice fail either plaque-forming