Palmitic acid conjugation of a protein antigen enhances major histocompatibility complex class II-restricted presentation to T cells.

摘要: The effect on antigenicity of covalent attachment lipid groups to a protein antigen was investigated. Coupling palmitic acid ovalbumin (OVA) enhanced major histocompatibility complex (MHC) class II-restricted presentation most OVA-specific murine T-cell clones in vitro. palmitoylated localized the level synthetic peptide epitope, OVA 323-339. responses were more difficult block with anti-MHC II antibodies than native antigen. However, proliferation palmitoyl (p)-OVA and (n)-OVA blocked equally by anti-CD4 antibodies. Taken together, results suggest that conjugation leads formation lipopeptide epitope increased affinity binding MHC and/or receptor (TcR). These have implications for design vaccines.