Determination of molecular weights and Stokes' radii of non‐denatured proteins by polyacrylamide gradient gel electrophoresis. 1. An equation relating total polymer concentration, the molecular weight of proteins in the range of 104–106, and duration of electrophoresis

摘要: Untreated and processed gel plates of polyacrylamide (PAA) gradient gels were cut into strips perpendicularly to their length, the wet dry matter sections was determined. In untreated apparent matter, as well relative are a linear function length. gels, however, only concentration increases linearly with whereas square root The %T (content in polyacrylamide) calculated from matter. gradients used found be respect %T. Six different calibration proteins run time-dependent migration distances For each protein relationship exists between its distance (√D) logarithm electrophoretical duration (log t). this relation √D can substituted by PAA or (TM(%)). resulting straight lines seem intersect near zero time electrophoresis starting gradient. At any during (t» O), logs Stokes' radii Rs), molecular weights MW), correlated linearly. function, may replaced either TM (%). It is shown that correlation not subject time, although constants changed process electrophoresis. slopes (at constant voltage) related hyperbolic function. Regression obtained for times range concentration. A more general equation formulated which states - within certain experimental limitations — size proteins, distances,