Effect of Fluid Shear Stress on Cardiomyogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

摘要: Background and Aims Bone marrow mesenchymal stem cells (BMSCs) are a potential source of material for the construction tissue-engineered cardiac grafts because their to transdifferentiate into cardiomyocytes after chemical treatment or co-culture with cardiomyocytes. Recent evidence has shown that mechanical loads could regulate BMSC differentiation osteoblasts endothelial through various signaling pathways. We investigated whether fluid shear stress (FSS), which is load generated by flow, can rat (rBMSC) Methods rBMSCs were isolated from femur tibia using density gradient centrifugation combined adhesion method identified surface marker, proliferation character in vitro. Cultured without 5-azacytidine (5-aza) exposed laminar parallel plate-type device analyzed RT-PCR, immunocytochemistry, FACS Western-blotting cardiomyogenic differentiation. Results Appropriate FSS alone induced rBMSCs, as confirmed expression cardiomyocyte-related markers at both mRNA protein levels. Furthermore, when rBMSC cultures 5-aza, levels significantly increased degree suggestive synergistic interaction. Conclusions The results demonstrate an important factor affecting rBMSCs. This provides new avenue mechanistic studies cell approach obtain more committed differentiated cells.