Distinct roles for pbs21 and pbs25 in the in vitro ookinete to oocyst transformation of Plasmodium berghei

摘要: We have developed an in vitro culture system for early sporogonic stages of Plasmodium berghei, which can be used to study developmental events normally taking place the midgut infected mosquito. These include penetration insect cells by mature ookinete, transformation into oocysts and development latter, sustained through several rounds nuclear division. The system, based upon co-culture enriched ookinetes with established cell lines, was mutant lacking both Pbs21 Pbs25 surface proteins. Motility entry double knockout single are normal, but number successfully transforming expressing CSP protein substantially reduced. Finally, using yeast two-hybrid we also show that has capacity homodimerise as well form heterodimers Pbs21.