A DNA repair defect in Chinese hamster ovary cells affects V(D)J recombination similarly to the murine scid mutation.

摘要: Lymphocyte antigen receptor variable regions are encoded by gene segments that assembled the site-specific (diversity) joining (V(D)J) recombination process. We have assayed V-3 Chinese hamster ovary cell line, which has a double-strand DNA break repair (dsbr) defect, for ability to carry out V(D)J following transfection of constructs encode RAG-1 and RAG-2 proteins necessary confer activity non-lymphoid cells. The cells had substantially impaired undergo transiently introduced test substrates. Although these can initiate introducing endonucleolytic scissions at junctions signal sequences flanking coding sequences, they greatly rejoin sequences. Detailed characterization attempted joins recovered from indicated defect in is similar phenotype murine severe combined immunodeficient (scid) quite distinct found other dsbr mutant lines. Somatic complementation analyses between homozygous scid fibroblasts confirmed mutations fall into same genetic group.