Golgi apparatus and intraneuronal inclusions of anterior horn cells in amyotrophic lateral sclerosis: an immunohistochemical study.

摘要: We applied immunohistochemical techniques to study alterations of the Golgi apparatus, and determine possible relationships between this complex intraneuronal inclusions lower motor neurons patients with sporadic form amyotrophic lateral sclerosis (ALS). Spinal cords from normal individuals served as controls. Monoclonal antibodies zone β-COP protein were used. Immunoreactivity these was seen in frozen sections paraformaldehyde-fixed spinal cord tissue, but not formalin-fixed, paraffin-embedded specimens. The immunoreaction products granular structures, diffusely distributed glial cells. Although immunostaining some ALS reduced, fragmentation apparatus evident either antibody. Bunina bodies skein-like inclusions, characteristically found anterior horn cells patients, stained by epitopes nor Lewy body-like hyaline present cases reported be characteristic familial posterior column degeneration. These results suggest that components are directly incorporated into inclusions. However, possibility abnormal proteinaceous material may involved their genesis cannot ruled out.